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Thermo Fisher
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Thermo Fisher
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Journal: Scientific Reports
Article Title: Oxidative stress-mediated impairment of human trophoblast cell proliferation by zinc pyrithione exposure
doi: 10.1038/s41598-026-38895-9
Figure Lengend Snippet: Effects of ZPT on Apoptosis and Cell Cycle. ( A ) Representative flow cytometry scatter plots of Annexin V-FITC/PI staining after 72-hour treatment with ZPT. The four quadrants represent viable cells (Annexin V⁻/PI⁻), early apoptotic cells (Annexin V⁺/PI⁻), late apoptotic cells (Annexin V⁺/PI⁺), and necrotic cells (Annexin V⁻/PI⁺). ( B ) Representative flow cytometry histograms of PI staining showing DNA content after 72-hour ZPT treatment. ( C ) Quantitative analysis of late apoptosis rate. Data are from three independent experiments and expressed as mean ± SD. ** p < 0.01. ( D ) Quantitative analysis of cell cycle distribution. The percentages of cells in each phase (G0/G1, S, G2/M) are summarized. Data are from three independent experiments and expressed as mean ± SD. ns: not significant.
Article Snippet: The pellet was resuspended in ice-cold PBS, followed by the addition of 1 mL
Techniques: Flow Cytometry, Staining